Anti-TTF1 - Rabbit (WRAB-1231)使用說明
產品信息
品牌 | Seven Hills Bioreagents |
貨號 | WRAB-1231 |
規(guī)格 | 100 µL |
宿主 | 兔 |
反應種屬 | 人、鼠 |
克隆類型 | 多克隆 |
偶聯物 | 無 |
緩沖液 | 0.01% sodium azide |
抗原 | 重組鼠TTF-1融合6*his蛋白 |
保存條件 | 儲存在-20℃,避免反復凍融 |
蛋白背景 | 甲狀腺轉錄因子-1(TTF-1)是同源域轉錄因子家族的成員,并且在調節(jié)甲狀腺,肺和腦內表達的基因中起作用。 |
產品使用
實驗應用 | 建議稀釋倍數 |
Western Blot | 1: 5000 |
Immunohistochemistry | 1:1000 - 1:2000 |
說明:最佳稀釋倍數與濃度應由研究人員確認
應用實例
圖片為成年小鼠肺組織,用6%正常山羊血清預包埋,并用抗大鼠TTF-1兔血清(1:2000稀釋)染色。通過DAB(黑色)觀察TTF-1,并用核快紅(粉紅色)復染。放大倍數10倍。
參考文獻
1. Zhou L, Lim L, Costa RH, Whitsett JA. 'Thyroid transcription factor-1, hepatocyte nuclear factor-3beta, surfactant protein B, C, and Clara cell secretory protein in developing mouse lung.' J Histochem Cytochem. 1996 Oct;44(10):1183-93.
2. Stahlman MT, Gray ME, Whitsett JA. 'Expression of thyroid transcription factor-1(TTF-1) in fetal and neonatal human lung.' J Histochem Cytochem. 1996 Jul;44(7):673-8.
3. Khoor A, Whitsett JA, Stahlman MT, Olson SJ, Cagle PT. 'Utility of surfactant protein B precursor and thyroid transcription factor 1 in differentiating adenocarcinoma of the lung from malignant mesothelioma.' Hum Pathol. 1999 Jun;30(6):695-700.
4. deFelice M, Silberschmidt D, DiLauro R, Xu Y, Wert SE, Weaver TE, Bachurski CJ, Clark JC, Whitsett JA. 'TTF-1 phosphorylation is required for peripheral lung morphogenesis, perinatal survival, and tissue-specific gene expression.' J Biol Chem. 2003 Sep 12;278(37):35574-83.
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