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pSicoR pGK puro載體

發(fā)布時(shí)間：2013/8/2 點(diǎn)擊次數(shù)：2758

pSicoR pGK puro載體

型號	載體名稱	出品公司	載體用途
VMA0373	pSicoR pGK puro	Addgene	慢病毒載體

產(chǎn)品參數(shù):

Backbone size （bp）: 7700
Modifications to Backbone: Puromycin is expressed from this plasmid as a selectable marker. Puromycin and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
Cloning site 5': HpaI
Cloning site 3': XhoI
5' sequencing primer: mU6-F

載體抗性:

氨芐青霉素（Ampicillin）

載體描述:

pSicoR PGK Puro allows for conditional （Cre-Lox）, stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS OFF shRNA expression. See pSicoR and pSico PGK Puro for more information.

The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map for pSico and on the Jacks Lab

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